Material Event (Form 8-K)

Item 8.01. Other Events.

In preparation for the expected Phase 2 study of PALI-2108 for the treatment of patients with moderate to severe Ulcerative Colitis ("UC"), and in order to expedite the enrollment of patients (the "Study Enrollment"), on March 30, 2026, Palisade Bio, Inc. (the "Company") entered into a master services agreement (the "MSA") with Iterative Scopes, Inc. d/b/a Iterative Health ("IH"), pursuant to which IH has agreed to, among other things, assist the Company with the Study Enrollment.

On March 27, 2026 (the "Closing Date"), the Company issued and sold 1,536,885 shares (the "Shares") of common stock, par value $0.01 per share ("Common Stock"), of the Company to an affiliate of IH (the "Investor") for a total purchase price of $3.0 million, or $1.952 per Share (which represents the average closing price of the Common Stock on the Nasdaq Capital Market for the five trading days prior to the Closing Date), pursuant to a common stock purchase agreement, dated March 27, 2026, by and between the Company and the Investor. The Shares were offered and sold in a transaction exempt from registration under the Securities Act of 1933, as amended, in reliance on Section 4(a)(2) thereof.