Amendment to Annual Report (Form 10-K/A)

This Amendment No. 1 on Form 10-K/A (this "Amendment") to the Annual Report on Form 10-K of Monopar Therapeutics Inc. (the "Company") for the fiscal year ended December 31, 2025, initially filed with the Securities and Exchange Commission (the "SEC") on March 27, 2026 (the "Original Filing"), is being filed to correct a typographical error in the Original Filing in the date of the Report of Independent Registered Public Accounting Firm (the "Audit Report") included in Item 8.

This Amendment is being filed solely to change the date of the Audit Report from "March 27, 2025" to "March 27, 2026". This Amendment includes Item 8, "Financial Statements and Supplementary Data" in its entirety and without change from the Original Filing other than the addition of the date of the signature of BPM LLP on the Audit Report.

In addition, pursuant to the rules of the SEC, the exhibit list included in Item 15 of Part IV of the Original Filing has been amended to contain currently dated certifications from the Company's Chief Executive Officer and Chief Financial Officer, as required by Sections 302 and 906 of the Sarbanes-Oxley Act of 2002. The certifications of the Company's Chief Executive Officer and Chief Financial Officer are filed as exhibits to this Amendment.

Except for the foregoing amended information, this Amendment does not amend or update any other information contained in the Original Filing.