Material Agreement, Financial Obligation (Form 8-K)

On January 21, 2026, Stoke Therapeutics, Inc. (the "Company") entered into a new lease for its corporate headquarters and laboratory with NWALP PHOP Property Owner LLC, located at 245 Fifth Avenue, Waltham, Massachusetts (the "Lease Agreement"), in order to expand its office and laboratory space. The Lease Agreement premises include approximately 98,500 square feet. The Lease Agreement will expire on March 31, 2038, and includes two options to further extend the Lease for an additional five years at the then-prevailing market rate. The aggregate estimated base rent payments due over the term of the Lease Agreement is approximately $85.5 million. The Company will pay a security deposit of $3 million in the form of an irrevocable standby letter of credit, which may be reduced over time in accordance with the terms of the Lease Agreement.

The foregoing description of the Lease is qualified in its entirety by reference to the Lease Agreement, a copy of which will be filed as an exhibit to the Company's Quarterly Report on Form 10-Qfor the period ended March 31, 2026.

The information set forth above in Item 1.01 of this Current Report on Form 8-K regardingthe Lease Agreement is incorporated into this Item 2.03 by reference.