NIST - National Institute of Standards and Technology
10/04/2026 | Press release | Distributed by Public on 10/05/2026 03:20
Tunable protein expression strength with toehold exchange riboregulators
Published
October 4, 2026
Author(s)
Samuel Schaffter, Fernanda Piorino Macruz de Oliveira, Eugenia Romantseva
Abstract
Toehold exchange (THE) riboregulators are a class of RNA molecules that use toehold-mediated strand exchange (TMSE) to regulate protein translation. The mechanism of TMSE offers many design parameters for precisely tuning the strength of protein expression such as: (1) Modulating the concentration of reactants (transcriptional control), (2) Modulating the rate and extent of reaction to produce products (post-transcriptional control), and (3) Modulating the translation rate of the products (translational control). This Developer Note presents detailed, step-by-step protocols for executing translational control experiments for THE riboregulators in cell-free expression systems.
Citation
Nucleus Engineering
Pub Weblink
Pub Type
Websites
Nanobiotechnology, Engineering / synthetic biology, Cell-free systems, Biotechnology, Biometrology and Biomanufacturing
Citation
Schaffter, S. , Piorino Macruz de Oliveira, F. and Romantseva, E. (2026), Tunable protein expression strength with toehold exchange riboregulators, Nucleus Engineering, [online], https://devnotes.nucleus.engineering/ (Accessed October 5, 2026)
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NIST - National Institute of Standards and Technology published this content on October 04, 2026, and is solely responsible for the information contained herein. Distributed via Public Technologies (PUBT), unedited and unaltered, on October 05, 2026 at 09:20 UTC. If you believe the information included in the content is inaccurate or outdated and requires editing or removal, please contact us at [email protected]