Failure to Satisfy Listing Rule (Form 8-K)

Item 3.01. Notice of Delisting or Failure to Satisfy a Continued Listing Rule or Standard; Transfer of Listing.

On September 19, 2025, Klotho Neurosciences, Inc. (the "Company") received a delinquency notification letter (the "Notice") from the Nasdaq Stock Market LLC ("Nasdaq") due to the failure of the Company's common stock to maintain a minimum bid price of $1 per share for 30 consecutive business days as required by Nasdaq Listing Rule 5550(a)(2).

In accordance with Nasdaq Listing Rule 5810(c)(3)(A), the Company has been provided 180 calendar days, or until March 18, 2026, to regain compliance. To regain compliance, prior to March 18, 2026, the closing bid price of the Company's common stock must be at least $1 for a minimumof ten consecutive business days.

If the Company fails to timely regain compliance with Nasdaq Listing Rules, the Company's common stock will be subject to delisting from Nasdaq.